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KMID : 1024520070160040409
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Journal of the Environmental Sciences
2007 Volume.16 No. 4 p.409 ~ p.414
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Effects of Dimethipin on α-amylase Activity of Barley Seeds
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Lee Joon-Sang
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Abstract
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Effects of dimethipin on -amylase activity of barley seeds were investigated. In the treatments of dimethipin, the indexes of germination were reduced to 17% and 24 % respectively. After seed germination, dimethipin was added to germinated seedlings and then the seedlings were kept to measure seedling length under illumination for 7 days. In control, the length of seedling was 5.7 cm, but in the treatments of dimethipin and dimethipin, seedling lengths were 5.5 cm and 1.2 cm respectively. In the relationship between dimethipin concentrations and -amylase activities, there was a linear curve. The more dimethipin was added to the seeds, the more -amylase activities were inhibited. In the treatments of dimethipin and dimethipin, -amylase activities were reduced to 33% and 71% respectively. Dimethipin also inhibited -amylase activities increased by gibberellin and the content of soluble protein. Therefore, it could be suggested that dimethipin might inhibit directly the activities of hydrolysis enzymes including -amylase or the expression of -amylase genes as germination and seedling growth were severely disturbed.
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KEYWORD
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α-Amylase, Barley seeds, Dimethipin, Gibberellin
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